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MOLECULAR BIOLOGY: WORKING WITH RNA

NUCLEIC ACID PROBES

1. In Situ Hybridization to Drosophila Embryo Sections Using α-[35S]-Riboprobes
2. DNase I Footprinting
3. Fluorescent Probe Preparation from RNA for DNA Microarrays
4. Generation of a Probe for cDNA Cloning by PCR Using Peptide Sequence Information
5. Labeling DNA by Random Priming with Radioactive dCTP and the Klenow Fragment of DNA Polymerase
6. Labeling of DNA Probes using Primer Extension
7. Labeling Oligonucleotides
8. Labeling Oligonucleotides Using T4 Polynucleotide Kinase
9. Northern Blot Hybridization and Wash Using Ribonucleic Acid Probes (Riboprobes)
10. Radiolabeling of Oligonucleotides
11. Radiolabeling RNA Probes with Polynucleotide Kinase for Screening of cDNA Libraries
12. Random Primed Labeling of DNA
13. S1 Nuclease Mapping Using Single-Stranded Radiolabeled Oligonucleotide Probes
14. Ultraviolet (UV) Photochemical Crosslinking to Detect RNA Binding Proteins





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